# Diamond error running humann

**URL:** <https://forum.biobakery.org/t/diamond-error-running-humann/2609>\
**Category:** HUMAnN\
**Created:** [September 19, 2021, 4:51pm UTC](https://forum.biobakery.org/t/diamond-error-running-humann/2609 "2021-09-19T16:51:38Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![looooooog](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/looooooog/32/1034_2.png) [@looooooog](https://forum.biobakery.org/u/looooooog)\
**Post date:** [September 19, 2021, 4:51pm UTC](https://forum.biobakery.org/t/diamond-error-running-humann/2609/1 "2021-09-19T16:51:38Z")

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hello boys. I have problems when i use humann, when I use the example demo.fastq.gz, I can successfully get the result: three .tsv documents. However, when i use my metagenome sequence data .fq(it is 12G), I always get error from diamond, it only show the message error form diamond, and i can not finished the analysis, so anyone can help me? because i do not know the reason.

the following is the error message:

CRITICAL ERROR: Error executing: /home/z/miniconda3/envs/biobakery3/bin/diamond blastx --query /mnt/f/database/humann/WAM5\_humann\_temp/WAM5\_bowtie2\_unaligned.fa --evalue 1.0 --threads 1 --top 1 --outfmt 6 --db /mnt/f/database/uniref/uniref90\_201901b\_full --out /mnt/f/database/humann/WAM5\_humann\_temp/tmpm8rcuv\_r/diamond\_m8\_7q30ns20 --tmpdir /mnt/f/database/humann/WAM5\_humann\_temp/tmpm8rcuv\_r

Error message returned from diamond :  
diamond v2.0.11.149 (C) Max Planck Society for the Advancement of Science  
Documentation, support and updates available at [http://www.diamondsearch.org](http://www.diamondsearch.org)  
Please cite: [http://dx.doi.org/10.1038/s41592-021-01101-x](http://dx.doi.org/10.1038/s41592-021-01101-x) Nature Methods (2021)

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<div class="post-metadata">

**Author:** ![franzosa](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/franzosa/32/3511_2.png) [@franzosa](https://forum.biobakery.org/u/franzosa)\
**Post date:** [September 30, 2021, 6:13pm UTC](https://forum.biobakery.org/t/diamond-error-running-humann/2609/2 "2021-09-30T18:13:33Z")

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It looks like you have diamond 2.0 installed, however HUMAnN 3.0 is designed for use with the endpoint of the previous diamond lineage, which I believe was 0.9.36. You could install HUMAnN 3.0 into something like a conda environment in order to have access to both versions of the diamond software.