# Demo.fastq.gz fail to produce the result
**URL:** https://forum.biobakery.org/t/demo-fastq-gz-fail-to-produce-the-result/2833
**Category:** HUMAnN
**Created:** [November 25, 2021, 7:48am UTC](https://forum.biobakery.org/t/demo-fastq-gz-fail-to-produce-the-result/2833 "2021-11-25T07:48:23Z")
**Posts on this page:** 2
**Page:** 1
### Author:  [@w\_ceasea](https://forum.biobakery.org/u/w_ceasea)
#### Post date: [November 25, 2021, 7:48am UTC](https://forum.biobakery.org/t/demo-fastq-gz-fail-to-produce-the-result/2833/1 "2021-11-25T07:48:23Z")
Dear all,
Humann3 fail to re-produce the pathabundance and pathcoverage like the toturial in my machine by following command while the genefamilies is right:
humann -i demo.fastq.gz -o functions --diamond …/…/ --thread 4
**The part log of running is**
11/25/2021 03:25:33 PM - humann.utilities - DEBUG: Check software, metaphlan, for required version, 3.0
11/25/2021 03:25:48 PM - humann.utilities - INFO: Using metaphlan version 3.0
11/25/2021 03:25:48 PM - humann.utilities - DEBUG: Check software, bowtie2, for required version, 2.2
11/25/2021 03:25:48 PM - humann.utilities - INFO: Using bowtie2 version 2.3
11/25/2021 03:25:48 PM - humann.humann - INFO: Search mode set to uniref90 because a uniref90 translated search database is selected
11/25/2021 03:25:48 PM - humann.utilities - DEBUG: Check software, diamond, for required version, 0.9.36
11/25/2021 03:25:49 PM - humann.utilities - INFO: Using diamond version 0.9.36
11/25/2021 03:29:08 PM - humann.search.prescreen - INFO: Found g\_\_Bacteroides.s\_\_Bacteroides\_dorei : 57.96% of mapped reads
11/25/2021 03:29:08 PM - humann.search.prescreen - INFO: Found g\_\_Bacteroides.s\_\_Bacteroides\_vulgatus : 42.04% of mapped reads
11/25/2021 03:29:08 PM - humann.search.prescreen - INFO: Total species selected from prescreen: 2
g\_\_Bacteroides.s\_\_Bacteroides\_vulgatus: 1195 hits
g\_\_Bacteroides.s\_\_Bacteroides\_dorei: 1260 hits
unclassified: 252 hits
11/25/2021 03:29:53 PM - humann.humann - INFO: Total gene families after translated alignment: 559
11/25/2021 03:29:53 PM - humann.humann - INFO: Unaligned reads after translated alignment: 87.2000000000 %
**pathabundance.tsv**
/# Pathway demo\_Abundance
UNMAPPED 5736.8494011104
UNINTEGRATED 2558.6999771082
UNINTEGRATED|unclassified 38.3748531259
PWY-4203: volatile benzenoid biosynthesis I (ester formation) 13.3772872602
PWY-4203: volatile benzenoid biosynthesis I (ester formation)|unclassified 13.3772872602
**pathcoverage.tsv**
/# Pathway demo\_Coverage
UNMAPPED 1.0000000000
UNINTEGRATED 1.0000000000
UNINTEGRATED|unclassified 1.0000000000
PWY-4203: volatile benzenoid biosynthesis I (ester formation) 0.8361658544
PWY-4203: volatile benzenoid biosynthesis I (ester formation)|unclassified 0.6438577695
**My envirionment is as following:**
**huaman\_config**
HUMAnN Configuration ( Section : Name = Value )
database\_folders : nucleotide = /data/home/xjsjfengweibing/wanghao/wjk/blast/tools/humann-3.0.1/humann/data/chocophlan\_DEMO
database\_folders : protein = /data/home/xjsjfengweibing/wanghao/wjk/blast/tools/humann-3.0.1/humann/data/uniref\_DEMO
database\_folders : utility\_mapping = /data/home/xjsjfengweibing/wanghao/wjk/blast/tools/humann-3.0.1/humann/data/misc
run\_modes : resume = False
run\_modes : verbose = False
run\_modes : bypass\_prescreen = False
run\_modes : bypass\_nucleotide\_index = False
run\_modes : bypass\_nucleotide\_search = False
run\_modes : bypass\_translated\_search = False
run\_modes : threads = 1
alignment\_settings : evalue\_threshold = 1.0
alignment\_settings : prescreen\_threshold = 0.01
alignment\_settings : translated\_subject\_coverage\_threshold = 50.0
alignment\_settings : translated\_query\_coverage\_threshold = 90.0
alignment\_settings : nucleotide\_subject\_coverage\_threshold = 50.0
alignment\_settings : nucleotide\_query\_coverage\_threshold = 90.0
output\_format : output\_max\_decimals = 10
output\_format : remove\_stratified\_output = False
output\_format : remove\_column\_description\_output = False
**humann\_test**
Ran 186 tests in 92.333s
OK
**./diamond version**
diamond version 0.9.36
**metaphlan -v**
MetaPhlAn version 3.0.13 (27 Jul 2021)
---
### Author:  [@lauren.j.mciver](https://forum.biobakery.org/u/lauren.j.mciver)
#### Post date: [February 11, 2022, 9:53pm UTC](https://forum.biobakery.org/t/demo-fastq-gz-fail-to-produce-the-result/2833/2 "2022-02-11T21:53:22Z")
Hello, Thank you for the detailed post. I don’t see any errors in your run so I think it all looks okay. If you are using a different database type/version your results might differ slightly from those in the demo.
Thank you,
Lauren