# Creating toy custom database for DSL2 nextflow module development

**URL:** https://forum.biobakery.org/t/creating-toy-custom-database-for-dsl2-nextflow-module-development/2166
**Category:** MetaPhlAn
**Created:** [May 28, 2021, 8:00am UTC](https://forum.biobakery.org/t/creating-toy-custom-database-for-dsl2-nextflow-module-development/2166 "2021-05-28T08:00:47Z")
**Posts on this page:** 1
**Showing post:** 5

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### Author: ![fbeghini](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/fbeghini/32/81_2.png) [@fbeghini](https://forum.biobakery.org/u/fbeghini)
#### Post date: [June 3, 2021, 12:36pm UTC](https://forum.biobakery.org/t/creating-toy-custom-database-for-dsl2-nextflow-module-development/2166/5 "2021-06-03T12:36:24Z")

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> [@MGordon](#):
>
> `#avg_read_length 732.1818181818181`

I see here you are using as input a metagenome produced using long reads. MetaPhlAn is supposed to work with short read, however you can try using a local alignment instead the default end-to-end by running it with `--bt2_ps very-sensitive-local`.

> [@MGordon](#):
>
> When developing these nextflow modules It’s strongly recommended to use data available on the github repo for testing

We made available in the bioBakery tutorial a couple of subsampled HMP metagenomes for testing purposes, you can find it at [metaphlan3 · biobakery/biobakery Wiki (github.com)](https://github.com/biobakery/biobakery/wiki/metaphlan3#run-multiple-samples)

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