# Combined MTX/MGX analysis, contamination removal

**URL:** https://forum.biobakery.org/t/combined-mtx-mgx-analysis-contamination-removal/7099
**Category:** KneadData
**Created:** [May 28, 2024, 2:38pm UTC](https://forum.biobakery.org/t/combined-mtx-mgx-analysis-contamination-removal/7099 "2024-05-28T14:38:26Z")
**Posts on this page:** 1
**Page:** 1

<div class="post-metadata">

### Author: ![rackajuh](https://avatars.discourse-cdn.com/v4/letter/r/898d66/32.png) [@rackajuh](https://forum.biobakery.org/u/rackajuh)
#### Post date: [May 28, 2024, 2:38pm UTC](https://forum.biobakery.org/t/combined-mtx-mgx-analysis-contamination-removal/7099/1 "2024-05-28T14:38:26Z")

</div>

Hello,

I am trying to perform combined MTX/MGX analysis. When running the pipeline (Kneaddata/Humann), would it be more reasonable in case of the metatranscriptome to align it to the human genome or the human transcriptome, or even create a custom kneaddata database combining the two datasets. I would really appreciate some input. Thank you!

Best,  
Mate