# Absence of organism annotation in the genefaimly.tsv

**URL:** <https://forum.biobakery.org/t/absence-of-organism-annotation-in-the-genefaimly-tsv/1464>\
**Category:** HUMAnN\
**Created:** [December 21, 2020, 3:51pm UTC](https://forum.biobakery.org/t/absence-of-organism-annotation-in-the-genefaimly-tsv/1464 "2020-12-21T15:51:33Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Min-Ting](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/min-ting/32/538_2.png) [@Min-Ting](https://forum.biobakery.org/u/Min-Ting)\
**Post date:** [December 21, 2020, 3:51pm UTC](https://forum.biobakery.org/t/absence-of-organism-annotation-in-the-genefaimly-tsv/1464/1 "2020-12-21T15:51:33Z")

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Hello!  
It’s great to run the user-friendly bioBakery modules! I am new to the shotgun metagenomic field so please let me know if I didn’t describe clearly about my issues.

I ran Humann 3 for my trimmomatic trimmed fastq file (cat merged paired-end). When I took a look at the genefamily.tsv file, I found that there is no organism shown as described in the humann tutorial/manual ([https://github.com/biobakery/biobakery/wiki/humann3](https://github.com/biobakery/biobakery/wiki/humann3)). Also, the pathway abundance is 0 for unmapped and unintegrated. I was wondering if I missed something when I typed my codes?

Thanks ahead!

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**Author:** ![franzosa](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/franzosa/32/3511_2.png) [@franzosa](https://forum.biobakery.org/u/franzosa)\
**Post date:** [January 4, 2021, 7:45pm UTC](https://forum.biobakery.org/t/absence-of-organism-annotation-in-the-genefaimly-tsv/1464/2 "2021-01-04T19:45:55Z")

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Hmmm… this does sounds odd, especially having 0 unmapped reads. Can you tell us a bit more about your sample/metagenomes/reads and the command you used to analyze it?