# About the Maaslin2 category

**URL:** <https://forum.biobakery.org/t/about-the-maaslin2-category/24>\
**Category:** MaAsLin\
**Created:** [October 28, 2019, 6:42pm UTC](https://forum.biobakery.org/t/about-the-maaslin2-category/24 "2019-10-28T18:42:28Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![sagunmaharjann](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.biobakery.org/sagunmaharjann/32/168_2.png) [@sagunmaharjann](https://forum.biobakery.org/u/sagunmaharjann)\
**Post date:** [October 28, 2019, 6:42pm UTC](https://forum.biobakery.org/t/about-the-maaslin2-category/24/1 "2019-10-28T18:42:28Z")

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Name: [**MaAsLin2**](https://huttenhower.sph.harvard.edu/maaslin2)  
[User manual](https://bitbucket.org/biobakery/maaslin2) || [Tutorial](https://bitbucket.org/biobakery/biobakery/wiki/maaslin)

Description: MaAsLin2 is comprehensive R package for efficiently determining multivariable association between clinical metadata and microbial meta’omic features. MaAsLin2 relies on general linear models to accommodate most modern epidemiological study designs, including cross-sectional and longitudinal, and offers a variety of data exploration, normalization, and transformation methods.

#### **Citation**

Mallick H, Tickle TL, McIver LJ, Rahnavard G, Nguyen LH, Weingart G, Ma S, Ren B, Schwager E, Subramanian A, Paulson JN, Franzosa EA, Corrada Bravo H, Huttenhower C. **“Multivariable Association in Population-scale Meta’omic Surveys”.**

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**Author:** ![CelyTG](https://avatars.discourse-cdn.com/v4/letter/c/65b543/32.png) [@CelyTG](https://forum.biobakery.org/u/CelyTG)\
**Post date:** [August 9, 2022, 3:02pm UTC](https://forum.biobakery.org/t/about-the-maaslin2-category/24/2 "2022-08-09T15:02:54Z")

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Hello there I am Cely, I am new in the bioinformatics and multi-omics world. Recently, I am dealing with HUMAnN3.0 and I have generated the file.tsv of gene families, pathways abundance, and coverage, however, now I want to process my data, statistically with MaAsLin2 and I am not sure how to start. I have run the tutorial from [GitHub - biobakery/Maaslin2: MaAsLin2: Microbiome Multivariate Association with Linear Models](https://github.com/biobakery/Maaslin2), but I cannot run my own data, maybe I am doing something wrong with the command lines or with the sort of data row or columns.  
I will be grateful to you for any light, advice, or guidance that you can give me.